Genetic markers for high oleic acid content in plants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 16/40 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2653339

The present invention relates to a mutated nucleic acid sequence of a delta-12 oleate desaturase enzyme (FAD2)-encoding nucleic acid from a Cruciferae plant, wherein said mutated nucleic acid sequence comprises the non-conservative mutation of at least one nucleotide of at least one codon representing an amino acid selected from the group consisting of: - the amino acid at position 259 of the amino acid sequence of said FAD2, - the amino acid at position 216 of the amino acid sequence of said FAD2, - the amino acid at position 90 of the amino acid sequence of said FAD2, - the amino acid at position 116 of the amino acid sequence of said FAD2, - the amino acid at position 276 of the amino acid sequence of said FAD2.

L'invention concerne une séquence d'acide nucléique muté d'un acide nucléique codant l'enzyme delta 12 oléate désaturase (FAD2) chez une plante Cruciferae, ladite séquence d'acide nucléique muté comprenant la mutation non conservatrice d'au moins un nucléotide d'au moins un codon représentant un acide aminé choisi dans le groupe composé de : l'acide aminé en position 259 de la séquence d'acides aminés de FAD2, l'acide aminé en position 216 de la séquence d'acides aminés de FAD2, l'acide aminé en position 90 de la séquence d'acides aminés de FAD2, l'acide aminé en position 116 de la séquence d'acides aminés de FAD2, l'acide aminé en position 276 de la séquence d'acides aminés de FAD2.

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