C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) G01N 21/76 (2006.01)
Patent
CA 2637932
Methods for detecting and assaying for glyoxylate, include enzyme-based assays and/or assays for hydrogen peroxide following liberation of hydrogen peroxide from glyoxylate, are disclosed. In some embodiments, the invention is directed to methods for assaying for glyoxylate produced by the reaction of peptidylglycine alpha-amidating monooxygenase (PAM). The subject invention also concerns methods for assaying for the enzyme peptidylglycine alpha- amidating monooxygenase and/or its substrates. The detection of glyoxylate is indicative of the presence of PAM and/or its substrates. The subject invention also concerns methods for screening for peptide hormones, amidated fatty acids, any N-acyl-glycine or N-aryl-glycine conjugated molecule, and generally compounds having a glycine reside in free acid form and attached to a carbonyl group.
Cette invention concerne des procédés de détection et de dosage de glyoxylate, lesquels procédés comprennent des dosages à base d'enzymes et/ou des dosages de peroxyde d'hydrogène effectués après la libération de peroxyde d'hydrogène de glyoxylate. Dans certains modes de réalisation, cette invention concerne des procédés de dosage de glyoxylate produit par la réaction de peptidylglycine alpha-amidating monooxygénase (PAM). Cette invention concerne également des procédés de dosage de l'enzyme peptidylglycine alpha-amidating monooxygenase et/ou de ses substrats. La détection de glyoxylate révèle la présence de PAM et/ou de ses substrats. Cette invention concerne en outre des procédés de criblage d'hormones peptidiques, d'acides gras amidés, de toute molécule conjuguée de N-acyl-glycine ou de N-aryl-glycine et, d'une manière générale, de composés comportant un résidu glycine sous forme acide libre et fixé à un groupe carbonyle.
Carpenter Sarah E.
Consalvo Angelo P.
Mehta Nozer M.
Merkler Dave J.
Miller Duncan A.
Carpenter Sarah E.
Consalvo Angelo P.
Marks & Clerk
Mehta Nozer M.
Merkler Dave J.
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