Graphitic nanotubes in luminescence assays

Details Graphitic nanotubes in luminescence assays Graphitic nanotubes in luminescence assays

G - Physics

01

N

G01N 33/52 (2006.01) C07K 17/06 (2006.01) C12Q 1/25 (2006.01) C12Q 1/68 (2006.01) G01N 21/01 (2006.01) G01N 21/62 (2006.01) G01N 33/531 (2006.01) G01N 33/543 (2006.01) G01N 33/551 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2248893

Graphitic nanotubes, which include tubular fullerenes (commonly called "buckytubes") and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

L'invention concerne des nanotubes graphitiques, en particulier des fullerènes tubulaires (appelés communément "buckytubes") et des fibrilles qui sont rendues actives par substitution chimique, pour servir de supports solides pour des dosages par électrochimioluminescence. Les nanotubes graphitiques sont modifiés chimiquement par des biomolécules à groupes actifs, avant le dosage. L'association de complexes électrochimioluminescents du ruthénium avec les nanotubes modifiés par des biomolécules à groupes actifs permet la détection de molécules telles que les acides nucléiques, les antigènes, les enzymes et les substrats d'enzymes, par de multiples systèmes.

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