Heterologous expression of an aspergillus kawachi...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/30 (2006.01) C12N 9/34 (2006.01) C12P 7/06 (2006.01)

Patent

CA 2567612

The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular starch hydrolyzing (GSH) activity and a glucoamylase (GA) in a fermentation step which also comprises ethanologenic microorganisms at a temperature of 25 - 40~C to obtain a fermentation broth having 5 to 20% ethanol.

L'invention concerne une composition d'enzyme hydrolysant l'amidon granulé, cette composition contenant une alpha-amylase stable aux acides (asAA) développant une activité d'hydrolysation de l'amidon granulé. La présente invention porte également sur un procédé en une opération pour produire un alcool, selon lequel un substrat d'amidon granulé est mis en contact avec une alpha-amylase, laquelle est stable aux acides (asAA) et développe une activité d'hydrolysation de l'amidon granulé (GSH), ainsi qu'avec une glucoamylase (GA) lors d'une fermentation qui comporte également des micro-organismes d'éthanol à une température de 25 - 40 ·C pour obtenir un bouillon de fermentation contenant 5 à 20 % d'éthanol.

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