High sensitivity detection of peroxidase activity

Details High sensitivity detection of peroxidase activity High sensitivity detection of peroxidase activity

G - Physics

01

N

167/45, 150/15.1

G01N 33/53 (2006.01) C12Q 1/28 (2006.01)

Patent

CA 2000686

P-1356 HIGH SENSITIVITY DETECTION OF PEROXIDASE ACTIVITY ABSTRACT OF THE DISCLOSURE A new class of peroxidase substrates consists of o-diaminobenzenes having a substituent in the position. The new substrates may be used in immunoassays in which a peroxidase is the lable, or in assays for the peroxidase itself. Immunoassays using other enzyme labels which cause formation of hydrogen peroxide may be followed using the substrate of the invention by adding the substrate and a peroxidase to the assay fluid and detecting color formed as a result of oxidation of the substrate by the formed peroxide.

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