High throughput screening assay for the trpm5 ion channel

Details High throughput screening assay for the trpm5 ion channel High throughput screening assay for the trpm5 ion channel

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/705 (2006.01) C12N 15/11 (2006.01) C12Q 1/02 (2006.01) C12Q 1/56 (2006.01) C12Q 1/68 (2006.01) C40B 30/06 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01) G01N 33/567 (2006.01)

Patent

CA 2626852

There exists a need in the art for high throughput screening assays that can identify compounds that specifically modulate the activity of fast-acting ion channels, such as TRPM5. Current methods suffer from a lack of sensitivity, low throughput, and are labor intensive. The claimed methods provide fluorescent assays with an optical readout that gives rapid readout of the results, has a high signal to noise background ratio, are easy to use, can be modified for automation and miniaturization, and provide verification that a compound specifically modulates TRPM5.

Pour les besoins de la technique, l'invention concerne des essais de dépistage à haut rendement permettant d'identifier des composés qui modulent spécifiquement l'activité de canaux ioniques à action rapide, du type TRPM5. Les procédés existants pêchent par manque de sensibilité et par faible rendement, et ils sont exigeants en main-d'oeuvre. Les procédés décrits reposent sur des essais à fluorescence, fournissant une lecture optique qui assure une production rapide de résultats, sur la base d'un rapport élevé signal/bruit de fond ; enfin, ces procédés sont faciles à utiliser, on peut les modifier aux fins d'automatisation et de miniaturisation, et ils permettent de vérifier qu'un composé exerce une modulation spécifique de TRPM5.

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