Highly conserved genes and their use to generate...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2283458

This invention relates to a repertory of nucleic sequences usable for the detection and/or identification of a bacterial, fungal or parasitical species, genus, family or group. This repertory is created by amplifying the nucleic acids of a plurality of given species with given primers. From this repertory are derived species-, genus-, family- or group-specific oligonucleotides used as probes or primers. Also, universal probes or primers are derived from the same repertory. All these probes or primers can be used in conjunction with probes or primers specific to the detection of any antibiotic resistance gene and/or toxin gene, in kits or methods designed for the detection of a set of bacteria, fungi or parasites, in association or not with antibiotic resistance or toxin production.

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