Ice nucleation deficient microorganisms by genetic manipulation

Details Ice nucleation deficient microorganisms by genetic manipulation Ice nucleation deficient microorganisms by genetic manipulation

C - Chemistry, Metallurgy

12

N

195/1.38, 71/4.5

C12N 15/00 (2006.01) A01N 63/00 (2006.01) C12N 1/20 (2006.01) C12N 15/90 (2006.01)

Patent

CA 1217738

12 ICE NUCLEATION DEFICIENT MICROORGANISMS BY GENETIC MANIPULATION ABSTRACT OF THE DISCLOSURE Ice nucleation bacteria are modified in vitro to confer an ice nucleation deficient phenotype. Modification is accomplished by deletion, substitution, insertion, inversion, or transversion of a DNA segment within the gene locus responsible for the INA pheno- type. By limiting such mutations to the particular gene locus, the modified microorganisms are genetically stable and free from random mutations which might adversely affect their competitive fitness. The modified microorganisms are useful for prevention of frost damage to susceptible plant hosts.

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