C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/02 (2006.01) A61K 45/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/04 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01) G06F 19/00 (2006.01)
Patent
CA 2311927
The present invention relates to an improved method for the identification and optionally the characterization of interacting molecules designed to detect positive clones from the rather large numbers of false positive clones isolated by two-hybrid systems. The method of the invention relies on a novel combination of selection steps used to detect clones that express interacting molecules from false positive clones. The present invention further relates to a kit useful for carrying out the method of the invention. The present invention provides for parallel, high-throughput or automated interaction screens for the reliable identification of interacting molecules.
L'invention porte sur un procédé amélioré d'identification et facultativement de caractérisation de molécules interactives conçu pour distinguer les clones positifs d'avec le nombre relativement important de clones faussement positifs isolés à l'aide de systèmes di-hybrides usuels. Ledit procédé repose sur une nouvelle combinaison d'étapes de sélection servant à distinguer les clones exprimant des molécules interactives d'avec les clones faussement positifs. L'invention, qui porte également sur des trousses permettant d'en mettre en oeuvre le procédé, permet de filtrer en parallèle ou à fort débit les interactions en vue d'une identification fiable des molécules interactives.
Bancroft David
Lehrach Hans
Maier Elmar
Meier-Ewert Sebastian
Wanker Erich
Borden Ladner Gervais Llp
Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.v.
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