Identification of essential genes of aspegillus fumigatus...

Details Identification of essential genes of aspegillus fumigatus... Identification of essential genes of aspegillus fumigatus...

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) A61K 38/43 (2006.01) A61K 39/395 (2006.01) A61K 48/00 (2006.01) C07K 14/38 (2006.01) C07K 16/14 (2006.01) C07K 19/00 (2006.01) C12N 1/14 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12P 21/00 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2445179

The present invention provides nucleotide sequences, methods and compositions that enable the experimental determination as to whether any gene in the genome of Aspergillus fumigatus is essential, and whether that gene is required for virulence or pathogenicity. The methods involve the construction of genetic mutants in which a target gene is placed under conditional expression. The identification of essential genes and those genes critical to the development of virulent infections, provides a basis for the development of screens for new drugs against Aspergillus fumigatus. The present invention further provides Aspergillus fumigatus genes that are essential and are potential targets for drug screening. The nucleotide sequence of the target genes can be used for various drug discovery purposes, such as expression of the recombinant protein, hybridization assay and construction of nucleic acid arrays. The uses of proteins encoded by the essential genes, and genetically engineered cells comprising modified alleles of essential genes in various screening methods are also encompassed by the invention.

L'invention concerne des séquences nucléotides, des procédés et des compositions permettant la détermination expérimentale sur la question de savoir si un gène quelconque dans le génome d'Aspergillus fumigatus est essentiel, et si ce gène est requis pour sa virulence et sa pathogénicité. Le procédé implique la construction de mutants génétiques, selon laquelle un gène cible est placé sous expression conditionnelle. L'identification de gènes essentiels et des gènes critiques pour le développement d'infections virulentes, constitue une base permettant le développement de criblage pour de nouveaux médicaments contre l'Aspergillus fumigatus. L'invention concerne en outre des gènes d'Aspergillus fumigatus qui sont essentiels et qui sont des cibles potentiels pour le criblage de médicaments. La séquence nucléotide des gènes cibles peut être utilisée pour diverses recherches de médicaments, par exemple, expression de protéine recombinante, test d'hybridation et construction de séries d'acides nucléiques. Enfin, l'invention se rapporte également aux utilisations de protéines codées par les gènes essentiels, et les cellules génétiquement manipulées comprenant des allèles modifiés de gènes essentiels dans différents procédés de criblage.

Affiliated with

Also associated with

Rating

Identification of essential genes of aspegillus fumigatus... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Identification of essential genes of aspegillus fumigatus..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Identification of essential genes of aspegillus fumigatus... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1575043