Immunoassay for nonenzymatically glucosylated proteins and...

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G01N 33/566 (2006.01) A61K 39/385 (2006.01) A61K 39/395 (2006.01) G01N 33/543 (2006.01) G01N 33/66 (2006.01) G01N 33/68 (2006.01) G01N 33/72 (2006.01)

Patent

CA 1206877

ABSTRACT OF THE DISCLOSURE An immunoassay method and reagent system for determining nonenzymatically glucosylated proteins and protein fragments in a biological fluid based on the specific binding of such proteins and fragments with anti(Amadori-rearranged glucose), e.g., antibodies which selectively recognize the rearranged deoxyfructose form of glucose resulting when proteins are nonenzymati- cally glucosylated. The antibodies are raised against an immunogen comprising an immunogenic carrier material bearing 1-deoxy-1-fructosyl residues or conformers of such residues. Measurement of nonenzymatically gluco- sylated proteins and fragments thereof provides a use- ful index of blood glucose levels.

436474

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