Immunoassay on a preblocked solid surface

Details Immunoassay on a preblocked solid surface Immunoassay on a preblocked solid surface

G - Physics

01

N

167/44

G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2000685

A method far immunoassay of a viral antigen is performed on a membrane precoated with an inert protein. Nonimmunological capture of antigen takes place by absorption onto the coated membrane. Captured antigen binds to a tracer which includes a label conjugated to a specific antibody, the inert protein concomitantly inhibiting nonspecific binding of tracer. The label may be an enzyme which converts a substrate to a detectable product or converts a blocked inhibitor to an inhibitor whereby a second enzyme is inhibited from converting a substrate o a product. The invention includes a kit of materials for performing an assay in accordance with the method of the invention.

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