Improved method for the biosynthesis of vitamin e

Details Improved method for the biosynthesis of vitamin e Improved method for the biosynthesis of vitamin e

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A01H 5/00 (2006.01) A01H 5/08 (2006.01) A01H 13/00 (2006.01) A01H 15/00 (2006.01) A23K 1/14 (2006.01) A23K 1/16 (2006.01) A23L 1/29 (2006.01) A23L 1/302 (2006.01) C12N 1/21 (2006.01) C12N 5/04 (2006.01) C12N 9/16 (2006.01) C12N 15/29 (2006.01) C12N 15/52 (2006.01) C12N 15/66 (2006.01) C12N 15/67 (2006.01) C12N 15/74 (2006.01) C12N 15/79 (2006.01) C12N 15/82 (2006.01) C12P 17/06 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2422760

The invention relates to improved methods for the biosynthesis of vitamin E. Said methods are characterized by the inhibition of the catabolization of homogentisate to maleylacetoacetate and fumarylacetoacetate and then to fumarate and acetoacetate. The invention also relates to the combination of this inhibition with methods that increase the provision of homogentisate or that promote the conversion of homogentisate to vitamin E. The invention further relates to nucleic acid constructs and vectors, which can be used to implement the inventive methods, in addition to transgenic plant organisms produced from said constructs and vectors.

L'invention concerne des procédés améliorés de biosynthèse de la vitamine E. Ces procédés se caractérisent par une inhibition de la dégradation de l'homogentisate en maleylacétoacétate et en fumarylacétoacétate, puis en fumarate et en acétoacétate. L'invention concerne également la combinaison de cette inhibition avec des procédés qui augmentent la mise à disposition de l'homogentisate ou qui favorisent la conversion de l'homogentisate en vitamine E. L'invention concerne enfin des constructions d'acides nucléiques et des vecteurs qui peuvent être utilisés pour la mise en oeuvre des procédés selon l'invention, ainsi que des organismes végétaux transgéniques produits à partir de ces constructions et de ces vecteurs.

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