C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/02 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01)
Patent
CA 2467142
A method for the recombinant production of a desired polypeptide by expressing a nucleic acid encoding said polypeptide in a microbial host cell, forming in the cytoplasm of said host cell inclusion bodies containing said polypeptide, and isolating, solubilizing and naturing said polypeptide, characterized in that after fermentation the host cell or the host cell content is incubated at a temperature of 40°C or higher for at least 10 minutes and subsequently insoluble polypeptide is isolated from the host cell, this method providing an improved yield in inclusion bodies containing the desired polypeptide.
Gruenbeck Rainer
Kopetzki Erhard
Popp Friedrich
F. Hoffmann-La Roche Ag
Gowling Lafleur Henderson Llp
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