Improving post-thaw survival of cryopreserved biological...

A - Human Necessities – 01 – N

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A01N 1/02 (2006.01)

Patent

CA 2537938

The present invention relates to a method for improving post-thaw survival of cryopreserved biological material comprising applying hydrostatic pressure to said biological material; keeping the said biological material at the hydrostatic pressure for a predetermined time period; releasing the hydrostatic pressure; and freezing the said biological material using any protocol applicable thereto. The invention also relates to the use of a pressurizing device for the pretreatment of a biological material that is to be cryopreserved, as well as to a pressurizing device for the pretreatment of a biological material that is to be cryopreserved, said device comprising a pressure chamber for receiving biological material, means to produce said pressure, and means to maintain said pressure in said chamber.

La présente invention concerne un procédé pour améliorer le taux de survie post-décongélation d'une substance biologique cryoconservée, consistant à appliquer une pression hydrostatique à ladite substance biologique; à maintenir ladite substance biologique à la pression hydrostatique pendant une durée prédéterminée; à libérer la pression hydrostatique; et à congeler ladite substance biologique à l'aide de n'importe quel protocole applicable à celle-ci. L'invention concerne également un dispositif de mise en pression et son utilisation pour le traitement préalable d'une substance biologique destinée à être cryoconservée, ce dispositif comportant une chambre de pression destinée à recevoir la substance biologique, des moyens pour produire ladite pression, ainsi que des moyens pour maintenir ladite pression dans ladite chambre.

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