In vitro induction of dopaminergic cells

A - Human Necessities – 61 – K

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A61K 35/30 (2006.01) C12Q 1/02 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2204629

The expression of thyrosine hydroxylase in neural cells in vitro can be induced by contacting neural cells with a culture medium comprising at least one member of the fibroblast growth factor family in combination with conditioned medium or molecules of the transforming growth factor beta family. The method induces neural cells obtained from normally non-dopaminergic neural tissue such as the striatum and the cortex to express tyrosine hydroxylase. The cells can be used to treat neurological disorders in patients requiring dopaminergic cells.

L'expression de tyrosine hydroxylase dans les cellules neurales peut être induite in vitro par mise en contact des cellules neurales avec un milieu de culture comportant au moins un membre de la famille des facteurs de croissance des fibroblastes combiné à un milieu conditionné ou à des molécules de la famille des facteurs de croissance transformants bêta. Le procédé induit des cellules neurales obtenues à partir de tissu neural normalement non dopaminergique tel que le corpus striatum et le cortex pour exprimer la tyrosine hydroxylase. Ces cellules peuvent servir au traitement de troubles neurologiques chez des patients nécessitant des cellules dopaminergiques.

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