Isolation of microbial protein with reduced nucleic acid...

Details Isolation of microbial protein with reduced nucleic acid... Isolation of microbial protein with reduced nucleic acid...

A - Human Necessities

23

J

195/128.4

A23J 1/18 (2006.01)

Patent

CA 1173389

Abstract of the Disclosure The present invention relates to a process for the isolation of protein from microbial cells. The microbial cells are subjected to a treatment to disrupt the cell walls thereby producing a cellular debris fraction and a fraction containing protein and nucleic acid. The two fractions are separated and the fraction containing the protein and nucleic acid is treated with a chelating agent at a pH in the range of 5.5 to 7Ø The resulting solution is passed through an anion exchange column which selectively adsorbs the nucleic acid. The protein is recovered from the resultant eluate.

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