L-ascorbic acid production in microorganisms

C - Chemistry – Metallurgy – 12 – P

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C12P 17/04 (2006.01)

Patent

CA 2183034

A process for the production of L-ascorbic acid by is disclosed. The process includes culturing organisms selected from the group consisting of organisms of the genus Prototheca and organisms of the species Chlorella protothecoides in a fermentation having a pH of less than about 6Ø The process further includes recovering L-ascorbic acid from the fermentation medium. A primary advantage of the disclosed process is that high concentrations of extracellular L-ascorbic acid are produced and at the pH of the disclosed process, significant degradation of extracellular L-ascorbic acid by oxidation does not occur. L-ascorbic acid has many known uses, such as for vitamin supplements.

L'invention porte sur un procédé permettant d'activer la production d'acide L-ascorbique par des microorganismes. Ce procédé comprend la culture d'organismes sélectionnés dans un groupe constitué d'organismes du genre Prototheca et d'organismes de l'espèce chlorella protothecoides dans un milieu en fermentation de pH inférieur à 6,0. Le procédé consiste, en outre, à récupérer de l'acide L-ascorbique dans le milieu en fermentation. L'un des principaux avantages est la production de concentrations élevées d'acide L-ascorbique extracellulaire et le fait qu'avec le pH spécifique au procédé, l'acide L-ascorbique extracellulaire ne subit pas de dégradation importante par oxydation. Cet acide a de nombreuses applications connues, notamment les suppléments vitaminiques.

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