Labeled reagents for use in immunoassays and fluorescent...

C - Chemistry – Metallurgy – 07 – C

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C07C 309/86 (2006.01) C07C 45/45 (2006.01) C07C 49/813 (2006.01) C07D 307/91 (2006.01) C07D 333/04 (2006.01) C07D 333/52 (2006.01) C07D 333/76 (2006.01) C07D 409/02 (2006.01) C07F 5/00 (2006.01) G01N 33/533 (2006.01)

Patent

CA 2187690

Disclosed are labeling reagents containing fluorescent compounds represented by the general formula (see fig. I) or (see fig. II) where R is a group capable of combining with proteins, Ar is a conjugated double bond system, and n is a whole number. These labeling reagents have high fluorescence emission intensities, give high synthesis yields, permit both solid-phase measurements and liquid-phase measurements in immunoassays, and require less measuring steps.

Réactifs de marquage, renfermant des composés fluorescents, représentés par la formule générale (voir fig. I) ou (voir fig. II), où R est un groupe pouvant se combiner avec des protéines, Ar un système à double liaison conjuguée, et n un nombre entier. Ces réactifs de marquage ont de fortes intensités d'émission de fluorescence, offrent des rendements de synthèse élevés, permettent des mesures tant en phase solide qu'en phase liquide dans les immunoessais et nécessitent moins d'étapes de mesure.

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