Labelling agents comprising boronic acid conjugates

C - Chemistry – Metallurgy – 07 – F

Patent

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Details

C07F 5/02 (2006.01) C09B 11/20 (2006.01) C09B 23/08 (2006.01) C09B 47/08 (2006.01) G01N 33/52 (2006.01) G01N 33/533 (2006.01) G01N 33/58 (2006.01) G01N 33/66 (2006.01) G01N 33/72 (2006.01)

Patent

CA 2096251

Labelling agents comprising non-proteinaceous boronic acid conjugates having absorption maxima at not less than 600 nm, said label being provided by e.g. an azine, triphenylmethane, cyanine or phthalocyanine dye, are useful in the estimation and quantification of cisdiols such as glycosylated haemoglobin, by virtue of the substantially total absence of overlap with the absorption spectrum of haemoglobin. Oxazine and thiazine dyes exhibiting similar absorption characteristics and containing other activated moieties are similarly useful labelling agents, especially in the presence of haemoglobin.

Agents de marquage comprenant des conjugués d'acide borique non-protéinique présentant une valeur maximale d'absorption supérieure ou égale à 600 nm, ledit marquage étant produit notamment par un colorant à base d'azine, de triphénylméthane, de cyanine ou de phthalocyanine. Ces agents sont utiles à l'estimation et à la quantification de cis-diols tels que l'hémoglobine glycosylée, en raison de l'absence pratiquement totale de chevauchement avec le spectre d'absorption d'hémoglobine. Des colorants à base d'oxazine et de thiazine présentant des caractéristiques d'absorption similaires et contenant d'autres fractions activées constituent pareillement des agents de marquage utiles, en particulier en présence d'hémoglobine.

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