Lactic acid-producing yeast cells having nonfunctional l-or...

Details Lactic acid-producing yeast cells having nonfunctional l-or... Lactic acid-producing yeast cells having nonfunctional l-or...

C - Chemistry, Metallurgy

12

N

C12N 1/18 (2006.01)

Patent

CA 2631036

Yeast cells having an exogenous lactate dehydrogenase gene ae modified by reducing L- or D-lactate:ferricytochrome c oxidoreductase activity in the cell. This leads to reduced consumption of lactate by the cell and can increase overall lactate yields in a fermentation process. Cells having the reduced L- or D- lactate:ferricytochrome c oxidoreductase activity can be screened for by resistance to organic acids such as lactic or glycolic acid.

Des cellules de levure comprenant un gène de lactate déshydrogénase exogène sont modifiées par réduction de l'activité L- ou D-lactate:ferricytochrome c oxydoréductase dans la cellule. Cela permet de réduire la consommation de lactate par la cellule et d'augmenter les rendements globaux de production de lactate dans un procédé de fermentation. Les cellules présentant une activité L- ou D-lactate:ferricytochrome c oxydoréductase réduite peuvent être criblées par résistance aux acides organiques tels que l'acide lactique ou glycolique.

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