Lipophilic quenching of viral inactivating agents

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) A61K 48/00 (2006.01) C07H 21/04 (2006.01) C12N 7/04 (2006.01) C12N 7/06 (2006.01)

Patent

CA 2360803

Methods and compositions for the inactivation and removal of contaminants of a biological composition are disclosed. The methods include the steps of: (a) contacting the biological composition with an inactivating agent including an aziridino moiety, such as ethyleneimine, an oligomer of tehyleneimine, or a haloderivative salt of either ethyleneimine or an oligomer of ethyleneimine, where a portion of the agent reacts with and inactivates the contaminant, and a portion of the agent remains unreacted; (b) contacting the product of step (a) with a lipophilic quenching agent including at least one quenching moiety attached to a lipophilic moiety, under conditions and for a time sufficient to allow the unreacted agent to bond covalently to the quenching moiety; and (c) separating the lipophilic quenching agent and the quenched inactivating agent from the biological composition.

L'invention concerne des procédés et des compositions permettant d'inactiver et d'extraire les contaminants d'une composition biologique. Ces procédés comprennent les étapes suivantes: (a) on met en contact la composition biologique avec un agent d'inactivation comprenant un groupe aziridino tel que l'éthylène-imine, un oligomère d'éthylène-imine, ou un sel dérivé halo de chaque élthylène-imine ou un oligomère d'éthylène-imine, une partie de cet agent réagit avec le contaminant de manière à l'inactiver et une autre partie de l'agent reste inaltéré; (b) on met le produit obtenu à l'étape (a) en contact avec un agent d'extinction lipophile comprenant au moins un groupe d'extinction fixé sur un groupe lipophile, dans des conditions, et pendant une durée permettant à l'agent inaltéré de former une liaison covalente avec le groupe d'extinction; et (c) on sépare l'agent lipophile et l'agent d'inactivation désactivé de la composition biologique.

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