M. tuberculosis rna polymerase alpha subunit

Details M. tuberculosis rna polymerase alpha subunit M. tuberculosis rna polymerase alpha subunit

C - Chemistry, Metallurgy

12

N

C12N 9/12 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2274098

The present invention provides isolated nucleic acids encoding RNA polymerase alpha subunit from M. tuberculosis, vectors comprising the nucleic acids, cells comprising the vectors, and methods for producing M. tuberculosis alpha subunit. The invention also provides in vitro and in vivo methods for high- throughput screening to identify inhibitors of M. tuberculosis RNA polymerase.

Cette invention, qui a trait à des acides nucléiques isolés codant une sous-unité alpha d'ARN-polymérase issue de M. tuberculosis, à des vecteurs comportant ces acides nucléiques et à des cellules renfermant ces vecteurs, concerne également des procédés de production de la sous-unité alpha issue de M. tuberculosis. Elle porte, de surcroît, sur des techniques in vitro et in vivo de criblages de sélection à haut rendement permettant d'identifier des inhibiteurs de l'ARN-polymérase issue de M. tuberculosis.

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