G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/53 (2006.01)
Patent
CA 2548407
Reagents and assays for kinase, phosphatase and protease enzyme activity which employ metal ion-phosphate ligand specific binding and fluorescent polymer superquenching are described. The assays provide a general platform for the measurement of kinase, phosphatase and protease enzyme activity using peptide and protein substrates. Reagents and assays based on DNA hybridization and reagents and assays for proteins which employ aptamers, antibodies and other ligands are also described.
L'invention concerne des réactifs et des essais destinés à l'activité d'enzyme kinase, phosphatase et protéase mettant en oeuvre une liaison spécifique de ions métalliques-ligands phosphate et de superextinction de polymères fluorescents. Les essais mettent en place une plate-forme générale destinée à la mesure de l'activité d'enzyme kinase, phosphatase et protéase, au moyen de substrats peptidiques et protéiques. L'invention concerne également des réactifs et des essais fondés sur l'hybridation de l'ADN et des réactifs et des essais destinés à des protéines mettant en oeuvre des aptamères, des anticorps et d'autres ligands.
Achyuthan Komandoor
Kumaraswamy Sriram
Kushon Stuart
Lu Liangde
Mcbranch Duncan
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Qtl Biosystems Llc
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