Method and apparatus for cryopreservation

Details Method and apparatus for cryopreservation Method and apparatus for cryopreservation

A - Human Necessities

01

N

A01N 1/02 (2006.01) C12N 1/04 (2006.01)

Patent

CA 2308250

A method of cryopreserving biological material, which method comprises providing a sample of the biological material, where a liquid phase of the sample includes at least one solute; lowering the temperature of the sample to a nucleating point at which ice nucleation can occur in the sample; effecting ice nucleation in the sample; and lowering the sample temperature from the nucleating point to the solidification point thereof, characterised in that the temparature lowering from the nucleating point to the solidification point is non linear, whereby the rate of change of solute concentration in the liquid phase decreases for more than 80 % of the time taken to lower the temperature from the nucleating point to the solidification point.

L'invention porte sur un procédé de cryoconservation de substance biologique, ce procédé consistant à produire un échantillon de substance biologique, la phase liquide de l'échantillon comprenant au moins un soluté; faire baisser la température de l'échantillon à un point de nucléation au niveau duquel la prise en glace peut se produire dans l'échantillon; effectuer la prise en glace dans l'échantillon; et faire baisser la température du point de nucléation au point de solidification, cette baisse de température s'effectuant de manière non linéaire. Le taux de variation de la concentration du soluté dans la phase liquide décroît de plus de 80 % du temps pris pour faire baisser la température du point de nucléation au point de solidification.

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