Method and device for producing lipid vesicles

A - Human Necessities – 61 – K

Patent

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Details

A61K 9/127 (2006.01) B01J 13/02 (2006.01) A61K 7/00 (2000.01)

Patent

CA 2427640

The invention relates to a device for producing lipid vesicles, which is equipped with a line (1) for transporting a polar liquid phase, with a line (2) for transporting an organic liquid phase containing lipids, with a collecting receptacle (7) for accommodating produced lipid vesicles, and with means for conveying the liquid phases through lines (1) and (2). At at least one location, the outer side of line (1) forms a common contact surface with line (2) inside of which a common opening (3) is provided that permits the flow of liquid and joins the inside of line (2) to the inside of line (1). Lines (1) and (2) do not contain agitating or dispersing aids in the area of the opening (3). The invention also relates to a method for the careful production of lipid vesicles, wherein the lipid phase is sprayed under pressure perpendicular to the direction of flow of the polar phase and into the same, whereupon lipid vesicles having a narrow size distribution form spontaneously and without the action of mechanical agitating or dispersing aids.

L'invention concerne un dispositif permettant de produire des vésicules lipidiques. Ce dispositif comprend un conduit (1) pour transporter une phase liquide polaire, un conduit (2) pour transporter une phase liquide organique contenant des lipides, un récipient collecteur (7) destiné à recueillir les vésicules lipidiques produites, ainsi que des moyens pour acheminer les phases liquides dans les conduits (1) et (2). Ce dispositif est caractérisé en ce que le conduit (1) forme avec sa face extérieure au niveau d'au moins un point une surface de contact commune avec le conduit (2), comportant une ouverture commune (3) laissant passer le liquide et reliant l'intérieur du conduit (2) à l'intérieur du conduit (1). Au niveau de l'ouverture (3), les conduits (1) et (2) sont exempts d'auxiliaires d'agitation ou de dispersion. L'invention concerne également un procédé pour produire de façon contrôlée des vésicules lipidiques, consistant à introduire sous pression la phase lipidique dans la phase polaire perpendiculairement au sens d'écoulement de cette dernière, des vésicules lipidiques de répartition dimensionnelle étroite se formant spontanément et sans intervention d'auxiliaires mécaniques d'agitation ou de dispersion.

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