C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C40B 60/00 (2006.01) C40B 30/04 (2006.01)
Patent
CA 2644688
A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
Furusho Kenta
Kamagata Yoichi
Kanagawa Takahiro
Koyama Osamu
Kurane Ryuichiro
Kankyo Engineering Co. Ltd.
Marks & Clerk
National Institute Of Advanced Industrial Science And Technology
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