Method for diagnostic marker development

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2737303

The invention relates to a method for the generation of unique molecular markers in existing breeding material by selecting a marker associated with a trait, identifying the existing variation at the nucleotide level within a set of markers within a germplasm and introducing a selectable marker by the introduction of one or more nucleotides at positions in a constant region of the marker by targeted nucleotide exchange.

La présente invention concerne la génération de marqueurs moléculaires uniques dans du matériau de reproduction existant par sélection dun marqueur associé à un caractère, identification de la variation existante au niveau nucléotidique dans un ensemble de marqueurs dans du plasma germinatif et introduction dun marqueur sélectionnable par introduction dun ou plusieurs nucléotides à des positions dans une région constante du marqueur par échange de nucléotides ciblé.

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