Method for dissociation of cells

Details Method for dissociation of cells Method for dissociation of cells

A - Human Necessities

23

J

A23J 3/20 (2006.01) A23K 1/00 (2006.01) C12N 1/06 (2006.01) C12P 19/04 (2006.01)

Patent

CA 2535546

Disclosed is a method for the dissociation of cells. Cells are processed under conditions of pH, temperature, and shear to thereby yield a mixture of cell wall ghosts and cytoplasm. Preferably, the cells are jet cooked at an alkaline pH to form an intermediate mixture, and the intermediate mixture is subsequently jet cooked. Generally, the cells become dissociated, whereby at least one separate cell wall component is substantially separate from the dissociated cell walls.

La présente invention concerne un procédé de dissociation cellulaire selon lequel les cellules sont traitées dans des conditions de pH, de température et de cisaillement telles que l'on obtient un mélange de parois cellulaires fantômes et de cytoplasme. De préférence, on cuit les cellules au jet de vapeur à un pH alcalin afin de former un mélange intermédiaire, et l'on soumet ensuite le mélange intermédiaire à une cuisson au jet de vapeur. Généralement, les cellules se dissocient, de manière qu'au moins un composant de paroi cellulaire séparé se trouve sensiblement séparé des parois cellulaires dissociées.

Affiliated with

Also associated with

Rating

Method for dissociation of cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for dissociation of cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for dissociation of cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1810654