Method for extracting a protein from milk

Details Method for extracting a protein from milk Method for extracting a protein from milk

A - Human Necessities

23

J

A23J 1/20 (2006.01) A61K 35/20 (2006.01) B01D 15/32 (2006.01) B01D 15/36 (2006.01)

Patent

CA 2673583

The invention relates to a method for extracting a protein from milk, having at least one hydrophobic pocket and a negative charge to the natural pH of milk, that comprises the following steps: a) skimming and delipidation of the milk; b) passing the delipidated and skimmed fraction containing said protein on a chromatographic substrate on which is grafted a ligand having both a hydrophobic characteristic and a ionic characteristic in pH conditions enabling the protein to be retained on said substrate, the pH being higher than 4.6; c) elution of the protein; d) purification of the eluded fraction by removing the milk proteins from said eluded fraction; and e) recovering said protein.

L'invention concerne un procédé d'extraction d'une protéine présente dans du lait, présentant au moins une poche hydrophobe et une charge négative au pH naturel du lait, comprenant les étapes suivantes de: a) Ecrémage et délipidation dudit lait, b) Passage de la fraction délipidée et écrémée contenant ladite protéine sur un support chromatographique sur lequel est greffé un ligand présentant à la fois un caractère hydrophobe et un caractère ionique, dans des conditions de pH permettant à ladite protéine d'être retenue sur ledit support, le pH étant supérieur à 4,6, c) Elution de la protéine, d) Purification de la fraction éluée par élimination des protéines du lait de ladite fraction éluée, et e) Récupération de ladite protéine.

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