Method for gene diagnosis of bovine hsp 70 deficiency

Details Method for gene diagnosis of bovine hsp 70 deficiency Method for gene diagnosis of bovine hsp 70 deficiency

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) A61B 10/00 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12N 15/12 (2006.01) C12P 19/34 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2433624

A method for gene diagnosis of bovine Hsp70 deficiency, which comprises (a) a step of obtaining a bovine nucleic acid sample, (b) a step of subjecting the nucleic acid sample obtained in step (a) to a gene amplification reaction to obtain a nucleic acid fragment in which a region including a mutation site likely to be present in bovine Hsp70 gene is amplified, and (c) a step of examining the presence of mutation on the nucleic acid fragment in step (b), the region including the mutation site being a region including 1997-11030 position of a base sequence shown in SEQ ID No. 1 of SEQUENCE LISTING in a base sequence of bovine Hsp70 gene. According to the invention, bovine Hsp70 deficiency and its carrier can be detected and diagnosed by a genetic engineering method easily and quickly, and a kit used for this purpose is provided.

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