Method for generating immortal dendritic cell lines

C - Chemistry – Metallurgy – 12 – N

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C12N 5/10 (2006.01) C12N 5/06 (2006.01) C12N 15/10 (2006.01) C12N 15/85 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2429952

Immortalized mammalian dendritic cell lines are provided that have characteristics of functional, mature dendritic cells; defined by surface phenotype, constitutive and inducible gene expression patterns, and functional effects on T lymphocytes. The cell lines are capable of continuous in vitro proliferation for extended periods of time. Of particular interest is the ability of the cell lines to present antigen in a stimulatory manner to naïve T cells. The dendritic cell lines are useful in gene discovery, the generation of dendritic cell subset-specific antibodies and probes; defining stages of dendritic cell differentiation and maturation; for induction of antigen specific stimulation of lymphocytes; for dissection of antigen specific anergy or tolerance in lymphocytes; for characterization of dendritic cell-natural killer cell interactions; for in vitro and in vivo analyses of dendritic cell- endothelial cell interactions; and for screening assays.

L'invention concerne des lignées cellulaires dendritiques mammaliennes rendues immortelles, qui présentent les caractéristiques de cellules dendritiques fonctionnelles parvenues à maturité, définies par le phénotype de surface, les motifs d'expression géniques constitutifs et inductibles, et les effets produits sur les lymphocytes T. Les lignées cellulaires en question sont capables de prolifération continue in vitro durant des périodes prolongées. La capacité de ces lignées à présenter un antigène de façon stimulante à des lymphocytes T naïfs est particulièrement intéressante. Lesdites lignées sont utiles dans les applications suivantes: recherche génique, élaboration d'anticorps et de sondes spécifiques à des sous-ensembles de cellules dendritiques, définition des étapes de la différenciation et de la maturation des cellules dendritiques, induction d'une stimulation des lymphocytes spécifique aux antigènes, dissection d'anergie ou de tolérance spécifique aux antigènes dans les lymphocytes; caractérisation d'interactions de cellules dendritiques et de cellules NK, analyses in vitro et in vivo des interactions de cellules dendritiques et de cellules endothéliales, et essais de criblage.

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