Method for identifying translationally regulated genes

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01) C12N 5/10 (2006.01) C12N 9/50 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2271068

A method for identifying translationally regulated genes includes selectively stimulating translation of an unknown target mRNA with a stress inducing element wherein the target mRNA is part of a larger sample of mRNA. The mRNA sample is divided into pools of translated and untranslated mRNA which are differentially analyzed to identify genes that are translationally regulated by the stress inducing element. A method for identifying gene sequences coding for internal ribosome entry sites includes inhibiting 5'cap-dependent mRNA translation in a cell, collecting a pool of mRNA from the cells, and differentially analyzing the pool of mRNA to identify genes with sequences coding for internal ribosome entry sites.

L'invention porte sur un procédé d'identification des gènes régulées par traduction, consistant à stimuler sélectivement la traduction d'un ARNm cible inconnu à l'aide d'un élément inducteur de stress, l'ARNm cible inconnu faisant partie d'un échantillon plus important d'ARNm. L'échantillon d'ARNm se divise en amas traduits et non traduits d'ARNm qui sont analysés différentiellement pour identifier les gènes régulés par traduction à l'aide de l'élément inducteur de stress. L'invention porte également sur un procédé d'identification des séquences géniques codant pour les sites internes d'entrée des ribosomes, consistant à inhiber dans une cellule la traduction de l'ARNm dépendant de la région 5' à coiffe, à recueillir un amas d'ARNm à partir des cellules, puis à effectuer une analyse différentielle de l'amas d'ARNm afin d'identifier les gènes présentant des séquences codant pour des sites internes d'entrée des ribosomes.

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