Method for introducing and expressing genes in animal cells

Details Method for introducing and expressing genes in animal cells Method for introducing and expressing genes in animal cells

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2219994

A method for introducing and expressing genes in animal cells is disclosed comprising infecting said animal cells with live invasive bacteria, wherein said bacteria contain a eukaryotic expression cassette encoding said gene. The gene may encode, e.g., a vaccine antigen, a therapeutica agent, and immunoregulatory agent or an anti-sense RNA or a catalytic RNA.

L'invention concerne un procédé d'introduction et d'expression de gènes dans des cellules animales consistant à infecter lesdites cellules animales avec des bactéries invasives vivantes, lesdites bactéries contenant une cassette d'expression eucaryote codant ledit gène. Le gène peut coder, par exemple, un antigène de vaccin, un agent thérapeutique, un agent immunorégulateur ou un ARN antisens ou un ARN catalytique.

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