Method for labeling individual cells

Details Method for labeling individual cells Method for labeling individual cells

G - Physics

01

N

G01N 33/50 (2006.01) C12Q 1/00 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2401270

A method is provided for the labeling of individual cells. Labeling is accomplished by coating a particle with at least one dye or nucleic acid sequence encoding a marker protein. The particle is then propelled toward the cell resulting in the particle contacting the cell for a time sufficient for the dye or nucleic acid to leave the particle and enter the cell. The present method allows for the differential labeling of individual cells within dense populations of cells.

L'invention concerne une méthode de marquage de cellules individuelles. Cette méthode consiste à appliquer un revêtement renfermant au moins un colorant ou une séquence d'acide nucléique codant une protéine-marqueur sur une particule. Cette particule est alors propulsée vers la cellule et entre en contact avec celle-ci pendant un laps de temps suffisant pour permettre au colorant ou à l'acide nucléique de quitter la particule et de pénétrer dans la cellule. La présente méthode permet un marquage différentiel de cellules individuelles dans des populations denses de cellules.

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