C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12P 19/34 (2006.01) G01N 21/31 (2006.01) G01N 33/50 (2006.01) G01N 33/52 (2006.01)
Patent
CA 2277089
The present invention relates to a method for measuring de novo T-cell production in humans, and more particularly to the assessement recent thymic emigrant (RTE) diversity in a T-cell sub-population of a patient by the detection of T-cell receptor .beta. chain DNA deletion circles (TCR.beta.DC) generated during TCR gene rearrangement of thymocytes in the thymus. The method comprises isolating a T-cell sub-population from a patient, extracting genomic DNA from the T-cell sub-population, amplifying the genomic DNA with a primer specific for a T-cell receptor .beta. chain DNA rearrangement deletion circle (TCR.beta.DC) family and detecting the TCR.beta.DC, the TRC.beta.DC being indicative of the presence of a RTE. The method assesses the quantitative and qualitative (diversity) intrathymic T-cell production by quantitating the relative frequency and diversity of RTEs within various sub-populations of circulating human T-cells. Such a method may be useful to study the diversity of the human thymic function and to monitor immune reconstitution of HIV patients.
Harris Jeffrey M.
Jenkins Morgan
Komanduri Krishna V.
Mccune Joseph M.
Poulin Jean-Francois
Institut de Recherches Cliniques de Montreal
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
The Regents Of The University Of California
Universite de Montreal
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