Method for modifying plant morphology, biochemistry and...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/53 (2006.01) A01H 5/00 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/06 (2006.01) C12N 15/82 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2412052

The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a plant cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. The invention also relates to novel plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising said sequences. The invention also relates to the use of said sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology. The invention also relates to the use of said sequences in the above-mentioned methods.The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins. The invention also relates to the use of said compounds as a plant growth regulator or herbicide.

L'invention concerne des procédés de stimulation de la croissance de racines et/ou le renforcement de la formation de racines latérales ou adventives et/ou de modifier le géotropisme de racines comprenant l'expression d'une oxydase cytokinine de plante ou d'une autre protéine réduisant le niveau des cytokinines actives dans des plantes ou des parties de plantes. L'invention concerne également de nouvelles protéines de plante, des séquences d'acide nucléique codant les protéines oxydases cytokinines ainsi que des vecteurs, des cellules hôtes, des cellules transgéniques et des plantes comportant lesdites séquences. La présente invention se rapporte aussi à l'utilisation desdites séquences en vue d'améliorer les caractéristiques associées aux racines comportant l'augmentation de la production et/ou le renforcement de la vigueur à la levée et/ou la modification des racines/du taux de pousse et/ou l'amélioration de la résistance à la verse et/ou l'augmentation de la tolérance à la sécheresse et/ou le développement in vitro de la propagation d'explants et/ou la modification du sort des cellules et/ou le développement de la plante et/ou la morphologie de la plante et/ou la biochimie de la plante et/ou la physiologie de la plante. L'invention concerne encore lesdites séquences dans les procédés susmentionnés. L'invention se réfère à des procédés d'identification et d'obtention de protéines et de composants interagissant avec les protéines oxydases cytokinines. Enfin, l'invention présente l'utilisation desdits composants comme régulateur de croissance de plantes ou herbicide.

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