Method for predicting the metabolism of drug in human liver...

G - Physics – 01 – N

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G01N 33/48 (2006.01) G01N 33/15 (2006.01)

Patent

CA 2577644

It is intended to provide a method of exactly presuming the metabolism of a drug in the human liver and its effect on the liver function. Namely, a method of presuming the metabolite of a test substance in the human liver and the liver function characterized by comprising administering the test substance to a nonhuman chimeric animal, which has human-origin hepatocytes having proliferative ability and in which the hepatocytes substantially have the liver functions of the chimeric animal, and a non-chimeric animal of the same species while protecting the animals from the attack by human complements produced by the hepatocytes, and then analyzing and comparing the metabolites of the chimeric animal and the non-chimeric animal.

L'invention concerne un procédé de présomption exacte du métabolisme d'un médicament dans le foie humain et de son effet sur la fonction hépatique. A savoir, un procédé de présomption du métabolite d'une substance test dans le foie humain et sur la fonction hépatique est caractérisé par le fait qu'il comprend l'administration de la substance test à un animal chimérique non humain, qui possède des hépatocytes d'origine humaine ayant une capacité à la prolifération et dans lequel les hépatocytes ont sensiblement les fonctions hépatiques de l'animal chimérique, et un animal non chimérique de la même espèce tout en protégeant l'animal de l'attaque par les compléments humains produits par les hépatocytes, et l'analyse et la comparaison des métabolites de l'animal chimérique et de l'animal non chimérique.

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