C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2047342
ABSTRACT OF THE DISCLOSURE A method is disclosed for producing a single stranded polydeoxynucleotide having two segments that are non-contiguous and complementary with each other. The method comprises the steps of providing in combination (1) a polynucleotide having two non-contiguous, non-complementary nucleotide sequences S1 and S2 wherein S2 is 5' of S1 and is at least ten deoxynucleotides long and (2) an extender probe comprised of two deoxynucleotide sequences, wherein the sequence at the 3'-end of the extender probe is hybridizable with S1 and the other of the deoxynucleotide sequences is homologous to S2 and (b) extending the extender probe along the polynucleotide. The method can also comprise providing in the combination a polydeoxynucleotide primer capable of hybridizing at least at its 3'-end with a nucleotide sequence complementary to S2 under conditions where (1) the extended extender probe is rendered single stranded, (2) the polydeoxynucleotide primer hybridizes with and is extended along the extended extender probe to form a duplex comprising extended primer, (3) the extended primer is dissociated from the duplex, and (4) the primer hybridizes with and is extended along the extended primer to form a duplex comprising extended primer, and repeating steps (3) and (4). The method finds particular application in the detection of polynucleotide analytes. F27090.WPF FF27090
Becker Martin
Rose Samuel
Ullman Edwin F.
Western Linda M.
Behringwerke Aktiengesellschaft
Johnson Douglas S. Q.c.
LandOfFree
Method for producing a polynucleotide for use in single... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for producing a polynucleotide for use in single..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing a polynucleotide for use in single... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1637050