Method for producing fv fragments in eukaryotic cells

Details Method for producing fv fragments in eukaryotic cells Method for producing fv fragments in eukaryotic cells

C - Chemistry, Metallurgy

12

N

530/15.06, 195/1

C12N 15/09 (2006.01) C07K 16/00 (2006.01) C07K 16/40 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1341411

The present invention provides a method for producing Fv fragments comprising transforming a eukaryotic host cell with a eukaryotic expression vector comprising an operon having a DNA sequence which encodes the variable domain only of an antibody light or heavy chain, culturing the transformed host cell under conditions which cause the protein encoded by the DNA sequence to be synthesized; and collecting the synthesized protein. The Fv fragments may be suitable for use as diagnostic or therapeutic agents.

596914

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