Method for producing gene transferred dendritic cells

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) A61K 39/00 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C12N 5/16 (2006.01)

Patent

CA 2544786

It is intended to provide a method of transferring a gene into a dendritic cell which comprises the step of contacting a minus strand RNA virus with the dendritic cell; a method of constructing a transgenic dendritic cell which comprises the step of contacting a minus strand RNA virus with the dendritic cell; a transgenic dendritic cell constructed by this method; and a method of activating a dendritic cell which comprises the step of contacting a minus strand RNA virus with the dendritic cell. Thus, it becomes possible to efficiently deliver a gene into a dendritic cell. A dendritic cell having an antigen gene or a cytokine gene transferred thereinto is useful as a vaccine.

Cette invention concerne : une méthode de transfert d'un gène dans une cellule dendritique, consistant à mettre en contact un virus à brin d'ARN négatif ; une méthode permettant de construire une cellule dendritique transgénique consistant à mettre en contact un virus à brin d'ARN négatif avec la cellule dendritique ; une cellule dendrtitiques transgénique obtenue par cette méthode ; et une méthode d'activation d'une cellule dendritique consistant à mettre en contact un virus à brin d'ARN négatif avec la cellule dendritique. Il est ainsi possible d'introduire efficacement un gène dans une cellule dendritique. Une cellule dendritique dans laquelle a été transféré un gène d'antigène ou un gène de cytokine est utile en tant que vaccin.

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