C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/21 (2006.01) C12N 15/09 (2006.01) C12P 7/56 (2006.01)
Patent
CA 2737429
Disclosed is a lactic-acid-producing Escherichia coli which has at least one gene including at least a sucrose hydrolase gene and selected from sucrose non-PTS genes (provided that a combination of a repressor protein (cscR), a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) and a combination of a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) are excluded) and is genetically so modified as to have a system for enhancing the production of lactic acid. Also disclosed is a method for producing lactic acid from a plant-derived sucrose-containing raw material by using the lactic-acid-producing Escherichia coli.
L'invention porte sur une Escherichia coli produisant de l'acide lactique qui a au moins un gène comprenant au moins un gène de saccharose hydrolase et choisi parmi les gènes de systèmes non-PTS d'utilisation du saccharose (à condition qu'une combinaison d'un répresseur (cscR), d'une saccharose hydrolase (cscA), d'une fructokinase (cscK) et d'une saccharose perméase (cscB) et une combinaison d'une saccharose hydrolase (cscA), d'une fructokinase (cscK) et d'une saccharose perméase (cscB) soient exclues) et qui est génétiquement modifiée de façon à avoir un système pour amplifier la production d'acide lactique. L'invention porte également sur un procédé pour la fabrication d'acide lactique à partir d'une matière première contenant du saccharose d'origine végétale à l'aide de l'Escherichia coli produisant de l'acide lactique.
Araki Tadashi
Miyazawa Daisuke
Mochizuki Daisuke
Morishige Takashi
Takahashi Hitoshi
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Mitsui Chemicals Inc.
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