Method for production of lactoferrin

A - Human Necessities – 23 – J

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A23J 1/20 (2006.01) A61K 38/00 (2006.01) C07K 1/18 (2006.01) C07K 14/79 (2006.01)

Patent

CA 2750701

The invention concerns a method for production of lactoferrin comprising at least the steps of : a) disposing of raw material that have not been treated at a temperature greater than 500C, b) submitting this raw material to a treatment in order to obtain a solution of Lactenin (LN) or Milk Basic Protein (MBP), c) submitting this LN or MBP solution to a step of purification on a cation exchange resin equilibrated with an acetate buffer at a pH between 4 and 9 and eluted with different buffer solutions containing different solute concentrations, d) and collecting a fraction containing Lactoferrin having more than 95 % of purity, having no polymers and substantially free of LPS, endotoxins and angiogenin. It also concerns the Lactoferrin obtained having more than 95 % of purity, substantially free of LPS, endotoxins and angiogenin with an iron saturation level comprised between 9% to 15 %.

La présente invention concerne un procédé de production de lactoferrine comprenant au moins les étapes consistant : a) à utiliser une matière première n'ayant pas été traitée à une température supérieure à 500 °C, b) à soumettre ladite matière première à un traitement afin d'obtenir une solution de lacténine (LN) ou de MBP, c) à soumettre cette solution de LN ou de MBP à une étape de purification sur une résine échangeuse de cations équilibrée au moyen d'un tampon acétate à un pH compris entre 4 et 9 et éluée au moyen de diverses solutions tampons présentant diverses concentrations en soluté, d) et à recueillir une fraction contenant de la lactoferrine d'une pureté supérieure à 95 %, ne comportant pas de polymères et pratiquement exempte de LPS, d'endotoxines et d'angiogénine. L'invention concerne également la lactoferrine obtenue, d'une pureté supérieure à 95 %, essentiellement exempte de LPS, d'endotoxines et d'angiogénine et présentant un coefficient de saturation en fer compris entre 9 et 15 %.

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