Method for protein n-myristoylation

Details Method for protein n-myristoylation Method for protein n-myristoylation

C - Chemistry, Metallurgy

12

N

195/1.27, 195/1.

C12N 15/54 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2036965

07-24(661)A METHOD FOR PROTEIN N-MYRISTOYLATION Abstract of the Disclosure There is disclosed a method for providing for the coexpression of N-myristoyltransferase and a protein substrate for said N-myristoyltransferase in E. coli comprising introducing into E. coli a dual plasmid system comprising (A) the isopropyl-.beta.-D- thiogalactopyranoside-inducible tac promoter, the g10-L ribosome binding site, a NMT gene, the kanamycin resistance gene and the p15A origin of replication in operable sequence and (B) the recA promoter, the g10-L ribosome binding site, a mammalian gene, the ampicillin resistance gene and the Col El origin of replication in operable sequence. This allows production of mammalian N-myristoylproteins or proteins containing covalently linked analogs of myristate with altered physical-chemical properties.

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