Method for purifying g-csf

Details Method for purifying g-csf Method for purifying g-csf

C - Chemistry, Metallurgy

07

K

C07K 14/535 (2006.01) A61K 38/19 (2006.01) C07K 1/16 (2006.01)

Patent

CA 2614820

The invention relates to a method for obtaining the recombinant granulocyte- colony-stimulating factor (G-CSF), said method involving at least one cation- exchange chromatography step and at least one hydrophobic interaction chromatography step, the two chromatography steps directly succeeding each other in any order. The invention especially relates to a method for purifiyng G-CSF using a mixture of G-CSF and other proteins, said method comprising two cation-exchange chromatography steps which are respectively carried out before and after a hydrophobic interaction chromatography step.

La présente invention concerne un procédé d'extraction du facteur recombinant stimulant les colonies de granulocytes (G-CSF). Ce procédé comprend au moins une chromatographie d'échange de cations et au moins une chromatographie d'interaction hydrophobe, ces deux étapes de chromatographie étant exécutées immédiatement l'une après l'autre dans n'importe quel ordre. L'invention concerne en particulier un procédé de purification du facteur G-CSF à partir d'un mélange de facteur G-CSF et d'autre protéines, ce procédé comprenant deux étapes de chromatographie d'échange de cations exécutées respectivement avant et après une chromatographie d'interaction hydrophobe.

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