C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/79 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2648009
Disclosed is a DNA construct comprising: a non-eukaryotic suppressor tRNA gene which contains no internal promoter sequence capable of functioning in an eukaryotic cell; and an eukaryotic or bacteriophage-derived promoter ligated to the 5'-terminus of the tRNA gene. Also disclosed is a method for synthesis of suppressor tRNA using the DNA construct. Further disclosed is a method for production of a protein having a non-natural amino acid integrated therein by using the DNA construct.
L'invention divulgue une construction d'ADN comprenant : un gène ARNt suppresseur non eucaryote ne contenant aucune séquence promotrice interne pouvant fonctionner dans une cellule eucaryote et un promoteur eucaryote ou a médiation par bactériophage ligaturé à la terminaison 5' du gène ARNt. L'invention divulgue également un procédé de synthèse de l'ARNt suppresseur au moyen de la construction d'ADN. Enfin, l'invention a pour objet un procédé de production d'une protéine à laquelle un acide aminé non naturel est intégré au moyen de la construction d'ADN.
Hino Nobumasa
Kobayashi Takatsugu
Mukai Takahito
Sakamoto Kensaku
Yokoyama Shigeyuki
Hino Nobumasa
Kobayashi Takatsugu
Mukai Takahito
Riken
Sakamoto Kensaku
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