Method for targeting transcriptionally active loci

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/90 (2006.01) A01K 67/00 (2006.01) A01K 67/027 (2006.01) C12N 15/00 (2006.01) C12N 15/79 (2006.01) C12N 15/85 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2449303

The present invention is a method of targeting promoter-less selection cassettes into transcriptionally active loci. In particular, the invention is a method for targeting promoter-less selection cassettes into transcriptionally active loci in stem cells or other eukaryotic cells with much greater efficiency than previously observed with other methods, thus reducing or eliminating the need to screen for targeted cells. The invention also encompasses the DNA targeting vectors, the targeted cells, as well as non- human organisms, especially mice, created from the targeted cells.

Cette invention se rapporte à un procédé servant à transformer par ciblage des cassettes de sélection dépourvues de promoteur en locus actifs sur le plan de la transcription. Cette invention concerne en particulier un procédé servant à transformer par ciblage des cassettes de sélection dépourvues de promoteur en locus actifs sur le plan de la transcription dans des cellules souches ou d'autres cellules eucaryotes avec une efficacité beaucoup plus grande que ce que l'on a pu préalablement observé avec d'autres procédés, réduisant ou éliminant ainsi la nécessité de cribler les cellules ciblées. Cette invention concerne également les vecteurs de ciblages d'ADN, les cellules ciblées ainsi que des organismes non humains, notamment de souris, créés à partir de ces cellules ciblées.

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