Method of analyzing expression of gene

Details Method of analyzing expression of gene Method of analyzing expression of gene

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2431170

A cDNA is prepared from mRNA. The prepared cDNA is subjected to incision with two different types of restriction enzymes. The sequence of a portion of each of the fragments obtained as a result of treatment is determined, and the fragments are classified on the basis of the determined sequence by using a primer set. At the same time, the fragments are amplified in a manner that the relative expression magnitude thereof continues to be reflected therein. When the fragments have been classified into a predetermined number of fractions, the respective fractions are subjected to electrophoresis, and a gene expression profile is produced from the results.

Un ADNc est préparé à partir d'ARNm et est coupé à l'aide de deux enzymes de restriction. La séquence d'une partie du fragment ainsi obtenu est reconnue, après quoi le fragment est classé avec un ensemble d'amorces, en fonction de la séquence. Ledit fragment est amplifié en même temps, alors que la dose d'expression relative est amplifiée et que les fractions classées d'une manière définie sont soumises respectivement à l'électrophorèse. En fonction de données d'électrophorèse, un profil d'expression génique est construit.

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