C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/20 (2006.01) C02F 3/34 (2006.01)
Patent
CA 2525314
The invention relates to: a method of treating liquid waste (liquid agricultural or industrial effluents or aquatic sites) which is loaded or polluted with tributyl phosphate (TBP), modified bacterial strains which can be used in the aforementioned treatment method, a method for monitoring changes in TBP pollution, and the device which is used to perform said treatment method. According to the invention, the liquid waste-treatment or -purification method essentially comprises: steps (1) consisting in bringing said liquid waste into contact with at least one non-sulphur purple photosynthetic bacterial strain which is resistant to TBP and which is selected from the group containing Rhodopseudomonas palustris (Rp. palustris), Rhodospirillum rubrum (Rs. rubrum), Rhodobacter capsulatus (Rb. capsulatus) or Rhodobacter sphaeroides (Rb. Sphaeroides) as well as the aforementioned modified bacterial strains in order to overexpress cytochrome P450 in conditions that enable the degradation of the TBP present in said waste, regardless of the initial TBP concentration; and (2) the recovery of the purified liquid effluents.
Procédé de traitement de déchets liquides (effluents industriels ou agricoles liquides ou sites aquatiques) chargés ou pollués en phosphate de tributyle (TBP), souches bactériennes modifiées susceptibles d'être utilisées dans ledit procédé de traitement, procédé de surveillance de l'évolution de la pollution par le TBP ainsi que dispositif de mise en .oelig.uvre dudit procédé de traitement. Ledit procédé de traitement ou d'épuration de déchets liquides comprend essentiellement les étapes de (1) mise en contact desdits déchets liquides avec au moins une souche de bactérie photosynthétique pourpre non- sulfureuse résistante au TBP sélectionnée dans le groupe constitué par Rhodopseudomonas palustris (Rp. palustris), Rhodospirillum rubrum (Rs. rubrum), Rhodobacter capsulatus (Rb. capsulatus) ou Rhodobacter sphaeroides (Rb. sphaeroides) ainsi que lesdites souches de bactéries modifiées pour surexprimer le cytochrome P450, dans des conditions permettant la dégradation du TBP présent dans lesdits déchets et ce quelle que soit la concentration initiale en TBP ; et (2) la récupération des effluents liquides purifiés.
Berne Cecile
Garcia Daniel
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