Method of detecting an intended nucleic acid in a sample

Details Method of detecting an intended nucleic acid in a sample Method of detecting an intended nucleic acid in a sample

C - Chemistry, Metallurgy

12

Q

195/1.12, 150/8.

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 1325761

ABSTRACT OF THE DISCLOSURE A method of detecting a nucleic acid which is expected to be contained in a sample, which method comprises subjecting the sample to the steps of (I) converting the nucleic acid to be detected in the sample into a "copied" nucleic acid which is of a single strand structure and is complementary to the sole or either of the strands of a "mother" nucleic acid, viz. the nucleic acid to be detected, or which is of a double strand structure such that one of the strands is complementary to the sole or either strand of the mother nucleic acid and the other strand has come from the mother nucleic acid or such that two of the strands are each complementary to each of the strands of the mother nucleic acid; and (II) determining the presence in the sample of the copied nucleic acid in the light of the specified nature given to the copied nucleic acid.

586786

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